Human MRPS12 (28S ribosomal protein S12, mitochondrial) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0857-48, ELK0857-96, ELK0857-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: RPS12; MRPS12; MRPS34; PSMD7; HTR1B

Assay Type: Competitive Inhibition

Sensitivity: 0.28 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: cell lysates and other biological fluids

Assay Length: 2h

Research Areas: Developmental science;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O15235

Research Use Only