Chicken PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0709-48, ELK0709-96, ELK0709-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Cyclin

Assay Type: Sandwich

Sensitivity: 1.37 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken PCNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken PCNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken PCNA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken PCNA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9DEA3

Research Use Only